LOCUS       sequence-118180-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gltA-udhA targeting guide RNA array E. coli , Low Phosphate 
            Induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  TITLE     SUPERSEDE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCASCADE-gltA2-udhA
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     178..195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(650..677)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(693..764)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(849..868)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa
       61 aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg
      121 aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc
      181 gagtcagtga gcgaggaagc ggaatatatc cctaggaaaa aaaaccccgc cctgtcaggg
      241 gcggggtttt tttttcggtt tatccccgct ggcgcgggga actcgattct tatacataaa
      301 agcaacagaa tggtaacggt ttatccccgc tggcgcgggg aactcgaact aataactgtc
      361 ccgaatgaat tggtcaatac ggtttatccc cgctggcgcg gggaactcga ggtggtacca
      421 gatcttttga attcaaagtt aaacaaaatt atttgtagag ggaaaccgtt gtggtctccc
      481 tgaatatacg cgttttatgc tcgcggggta acatgacatg ctcgaattac agaaaaataa
      541 cttttttgtt acatttgtaa gatagtaagg tgtcagaaag agttatccag caaccactca
      601 tagacctagg gcagcagata gggacgacgt ggtgttagct gtgtataaac gcagaaaggc
      661 ccacccgaag gtgagccagt gtgactctag tagagagcgt tcaccgacaa acaacagata
      721 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggatgatgtc
      781 tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat
      841 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta
      901 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag
      961 gcaccatact cactttt
//
