LOCUS       sequence-118184-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl5 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL5A
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(644..668)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(700..719)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(701..719)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(764..863)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(793..813)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctatcat ctaatcttaa gtcgtgacat catccattca
      121 agtccttggc acaatccctc gccagttaga gcacagcatg cctggatatg ccactggtga
      181 tctttaatag aagttagctt caaaaactgg gagatttctg ctacagtcat gcattcttta
      241 acatcttgtt tattagcaaa aatcagcaat ccagcttttc ttaggtcctc atgcgctaac
      301 attttataga gttcttctct agttacagaa atcctctctc tgtctgtact gtccacaaca
      361 actattacaa actctgtgtt agtatagtaa gtgttccagg aagaacgaag agattcttgg
      421 ccaccaatat cccacattag gaaacgtgta ttattaatca ctatctcttc tacattactt
      481 cctattgtag gagatgtatg tacaacttca ttcatagaaa attggtaaag aatggtagtt
      541 ttccctgcat tatccaaccc aacaatgata actttgtgct cctggtgatt gaacagtctc
      601 catattctag tgaagagaat tcccatggtt ctatctcctt ctaagcctgc ttttttgtac
      661 aaacttgttt gatcaagctt aactagccag cttgggtctc cctatagtga gtcgtattaa
      721 tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga
      781 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
      841 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
      901 gacttggaaa tccccgtgag tcaaccgcta tccac
//
