LOCUS       sequence-118186-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl5b with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL5B
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(642..666)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(698..717)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(699..717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(762..813)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(791..811)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac
       61 tttgtacaag aaagctgggt cctattatct cacaccaatc cgggaggtca tccactctag
      121 accttggcat aacccttctc ctgtgagagc acagcaggat tgaatgtgcc atggatgatc
      181 cttaattgaa ctaagggtga ggtatttcga gatttcagct gctgtcatac accctttcat
      241 atcctgttta tttgcaaaga taaggactgc agccttccgt aaatcctcat gagccaacat
      301 tctgtataat tcttcttttg taatagctag tcgttccctg tcaatgctat caacaacaag
      361 aatgatgaac tctgtatttg agtaatatgt gttccaggat gatcgcagag actcctgacc
      421 accaatatcc cacataagaa aatgagtgtt cttcacaact atttcttcaa cattgcttcc
      481 tatggttgga gaagtatgaa ccacttcatt cattaagaat tggtaaagaa tggtggtttt
      541 ccctgcatta tccagtccca ctataattac tttgtgttct tggttacaga agaggctcca
      601 cagtttggcg aagatcagcc ccatggttct atctccttct aagcctgctt ttttgtacaa
      661 acttgtttga tcaagcttaa ctagccagct tgggtctccc tatagtgagt cgtattaatt
      721 tcgataagcc agtaagcagt gggttctcta gttagccaga gagctctgct tatatagacc
      781 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
      841 ggaaagtccc gttga
//
