LOCUS       sequence-118196-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl8 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL8A
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(665..689)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(721..740)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(722..740)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(785..884)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(814..834)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctatcag cttctccgtg acttcgagtg ttgaataagc
      121 cactgtaggg tgatgtcaat gttgtccttt tctttgcaag agatggagta gcagcagatc
      181 tctcggtcct ggatggcaga cagattcatt ttctcaatca gctccttctc atccaatgct
      241 cccggaaggt ctcgcttgtt acccaggact aagaccggga tgccctgcag ctgaggtttg
      301 tccagtaggt tgtggagctc gttcttagag gcctcaatct tctcctggtc agcagcatcc
      361 accatgtaca cgatggcgct cactcctcgg cagtagcgct cccacatgct gcggaaacgc
      421 ggctgtcccc caatgtccca gagcttgata gtcacattcc ctttggtgat tttgcgcatg
      481 ttgaaaccca cggtggggat catgtcctcg ttgaactgtc ctgacgcgat cacgttgacg
      541 aaggtggtct tgcccgagta ctgaagcccg accagcgtga gctccatctc ctccttccag
      601 aatagggcct tgaaccagtc cagcagcttg ttgaacaaag cgatcatggt tctatctcct
      661 tctaagcctg cttttttgta caaacttgtt tgatcaagct taactagcca gcttgggtct
      721 ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc
      781 agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      841 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aaacaaaact
      901 ccccattgac gtcaatgggg tggagacttg gaaa
//
