LOCUS       sequence-118203-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl4D with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL4D
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(710..734)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(766..785)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(767..785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(830..881)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(859..879)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctatcac cgtctcttct tgccaccccg agctgccttc
      121 ttcctcttga ggatcatctc atagaggcgc tcaaggccct gctgcaggcc cagaccgtcc
      181 acagcgctgc agccttgcac atgagtgaga gtggcggctg ctagctctcg gactgccagc
      241 ctcttctcca cctcagcagc gctcagtgcc ccgggctggt cctgcttgtt ggccagcacc
      301 agcactggca cgccctggtt gtccgaggcc cggctgattc ggtgcaactc caccttggct
      361 tcctccagcc gctcagcctc cgcagcgtcc accacaaaca ctagaccgtc tgtccggcgg
      421 gtataagagc gccacagtgg tcgcagcttc tcctgccccc cgacgtccca cacttggaag
      481 gtgatgccac gcgatccccc gaggggcacc cggatcttct cggtgttgaa gcctttggtg
      541 gggacactct ggacaaactc cttgaacttg aggcggtaaa ggagggaggt ctttccagca
      601 gagtccagcc caatgaccac gacatgcagg gcttggaagt ggggcaagaa ggaggaggca
      661 gtgggcgcca tctcagtcaa gtggttcccc atggttctat ctccttctaa gcctgctttt
      721 ttgtacaaac ttgtttgatc aagcttaact agccagcttg ggtctcccta tagtgagtcg
      781 tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta
      841 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caa
//
