LOCUS       sequence-118207-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Howarth M, Takao K, Hayashi Y, Ting AY
  TITLE     Targeting quantum dots to surface proteins in living cells with 
            biotin ligase.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 May 24. 102(21):7583-8.
  PUBMED    15897449
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 May 24. 102(21):7583-8."
COMMENT     Sequence Label: pET21a-BirA
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(45..92)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     88..106
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(159..176)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggcgcgtcc cattcgccaa tccggatata gttcctcctt tcagcaaaaa acccctcaag
       61 acccgtttag aggccccaag gggttatgct agttattgct cagcggtggc agcagccaac
      121 tcagcttcct ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg
      181 agtgcggccg caagcttttt ttctgcacta cgcagggata tttcaccgcc catccagggt
      241 tttattattc catcctgctc aagtaataaa gccccctgtt tgtctattcc gcgtgaaatg
      301 ccaaatattt ctttatcacc aatgataagt ttcactgggc gattaataaa attatccagc
      361 ttttcccagc gcgacagata aggtgccaat ccttcttgtt cgaagagttc caacgcagca
      421 cgtaattcac gtattagcat ggccgccaac gtattacgat cgagattgat ccccgcttcc
      481 tgcagcgtga tccacccctg attaacgaca ctctcttcaa cacggcgcat tgccatgttg
      541 atcccggctc caatgactat ttgcgccgca tcgccagttt tgccagtcag ctccaccaga
      601 atgcctgcca gcttgcgatc ctgcagatag aggtcattag gccatttaac acgaacttta
      661 tctgcaccca gcttgcgtaa tacttccgcc atcacgatac cgataaccag acttaaacca
      721 atcgccgccg ccgggccttg ttccagacgc cagaacatcg acaaatataa gtttgcgcca
      781 aaaggcgaaa accatttccg accccggcga ccacggccag cctgctggta ttctgcaatg
      841 caagcatcgc ccgatttaag ctctccgata cgatcaagaa ggtactgatt cgtggagtca
      901 atcacaggca gcacggctac actaccgcca tccagctgac ccaatatctg tttagcatta
      961 agtaactgga taggctcagg caggctgtat cctttacccg gaacggtaaa gacatcaacg
     1021 ccccagtcac gcagtgtctg aa
//
