LOCUS       sequence-118208-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl14 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL14
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(681..705)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(737..756)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(738..756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(801..852)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(830..850)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac
       61 tttgtacaag aaagctgggt cctatcagtt ctgcttgaag aacgccaaag tgtctcctct
      121 tgatttcatg tggctcttca caaatccagt taatttcctg aacccctggg ccagcccctc
      181 ccctgtgagg gcacagcagg gttgcacata ccagttccgg tcactgcaaa gcttcttcac
      241 tttgaacatt ctggtgatgt cctcagcagt cagagctcca ggcatgtctt gtttgttggc
      301 taatagaaca acaggcacat ttttaatgtg ttcattcttc aaaatgtgct caaactgtct
      361 ctgagactct tccagtcgct gtttgtctgt actgtccaca acatacacca gcccatcggt
      421 gttctcacag taacagcccc aaacagttct cattttttcc tgtcctccaa catcccagac
      481 tgtgagtgaa aaattccttt ccaactcgat catttccaca ttgaaaccta ttgtagggat
      541 ggtggtaata tccttagcaa gctttaattt ataaaggaga gtagacttcc cagctgagtc
      601 aagtcccaaa agaagaactt gggcttgttt ggtttgcgga tttttagaac ccagcgaacc
      661 catggttcta tctccttcta agcctgcttt tttgtacaaa cttgtttgat caagcttaac
      721 tagccagctt gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg
      781 ggttctctag ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg
      841 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg t
//
