LOCUS       sequence-118209-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent indicator for PI(3,4,5)P3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Kawano F, Suzuki H, Furuya A, Sato M
  TITLE     Engineered pairs of distinct photoswitches for optogenetic control 
            of cellular proteins.
  JOURNAL   Nat Commun. 2015 Feb 24;6:6256. doi: 10.1038/ncomms7256.
  PUBMED    25708714
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-02-24"; volume: "6:6256. doi"; pages: " 
            10.1038/ncomms7256"
COMMENT     Sequence Label: pcDNA3.1_AktPH-mCherry
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     303..322
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    321..376
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     422..441
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             638..649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cataagagaa gagggacagc tatgactggg agtagtcagg agaggaggaa aaatctggct
       61 agtaaaacat gtaaggaaaa ttttagggat gttaaagaaa aaaataacac aaaacaaaat
      121 ataaaaaaaa tctaacctca agtcaaggct tttctatgga ataaggaatg gacagcaggg
      181 ggctgtttca tatactgatg acctctttat agccaacctt tgttcatggc agccagcata
      241 tgggcatatg ttgccaaact ctaaaccaaa tactcattct gatgttttaa atgatttgcc
      301 ctcccatatg tccttccgag tgagagacac aaaaaattcc aacacactat tgcaatgaaa
      361 ataaatttcc tttattagcc agaagtcaga tgctcaaggg gcttcatgat gtccccataa
      421 tttttggcag agggaaaaag atctcagtgg tatttgtgag ccagggcatt ggccacacca
      481 gccaccacct tctgataggc agcctgcacc tgaggagtga attgtaccga tgcggggagg
      541 cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca
      601 gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc
      661 agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccat ggaaaggacg
      721 tcagcttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc
      781 agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agg
//
