LOCUS       sequence-118211-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl11 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL11
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(695..719)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(751..770)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(752..770)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(815..866)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(844..864)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctatcaa gatctcttgc tgtctccgcg ctcagcccca
      121 tgggctctcg cctgcagaca catgcagctg cgagatttca ggaggctcca caggctctgc
      181 agggcctcgg gcagcccctc cccagtgagg gcactgcagc cccggagctc ccagcggtgg
      241 tcctggaatc tctctagact cagcctgttt ctgatcttaa gcagcggaag tgcatcaggt
      301 gcctcctgct tgttggccag caccaagaag gggacgccag ccatgttggg gtcgttcagg
      361 acttctgtga gctcagccgc cgactcgggt aagcgggctt catctgtgct gtccagtacg
      421 tacacgagga tatctgtgcc ttccagatag tccttccagc tggctctgag cggggcctgc
      481 cccccaacgt cccagagagt cagtgacacg tgcccaggag ctttcagagg ctccacgttg
      541 aaaccaacag tgggcagggt ctccaccagc tggtggccct tcagcttgta aaggagcgtg
      601 gtcttgcccg ccgagtccag gcccatcatc accacctggg cttccgcctt gtgacctctg
      661 gaattcacag aacccatggt tctatctcct tctaagcctg cttttttgta caaacttgtt
      721 tgatcaagct taactagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa
      781 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc
      841 gtacacgcct accgcccatt tgcgtca
//
