LOCUS       sequence-118216-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArfrp with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARFRP1
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(710..734)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(766..785)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(767..785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(830..881)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(859..879)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctactac gtgatgtccc tctgccgcgg cggccggtgc
      121 acattccgca cgacacactt caccatccac tcgatgccct cgcgcacccc tttgcctgtg
      181 agggccgagc aggcctgggt caggcaatcg cgcctgccga tcttgctggt gcagtcgctg
      241 aaggccgtct tgatgtcagg gattgagagg cacgtctcca catcctgctt gttggccagc
      301 accaagacgg ggacaccgca cagcgcctcg ctggtcacca ccttctcaaa cgcctgcttg
      361 gactcagcca gcctctcctc gtcggtggag tcaatgacgt agatgacgcc gtgacactcc
      421 gcataatact tgtcccacaa agactgcagc tcttcctgcc ctcctaagtc ccagaacatg
      481 agccgagcct ttcccacatc cacagtgccg atgtttaggc ccacggtggt ggtgattttg
      541 gatagactca tccccttgta gttcttgtta aatcgggttt tcgactgctc caggaaggtc
      601 gtcttcccag cattgtccag gcccaggatc aggatgcagt actcgtcctt ctgaaacatg
      661 tacttgtaca agcccgacag cagcgtgtac atggttctat ctccttctaa gcctgctttt
      721 ttgtacaaac ttgtttgatc aagcttaact agccagcttg ggtctcccta tagtgagtcg
      781 tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta
      841 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatg
//
