LOCUS       sequence-118217-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 448 / 473, 
            Emission: 500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(124..444)
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     regulatory      441..450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc
      121 cgcttatcca tccagctgca ctccgatgaa atactggagc tcacccttct ggtccctcat
      181 gggctggagg tgcaggaggt tccagaattt cttcccgctt tttgtgtagt ttatcagctg
      241 cacggtaatc tcgcgctgat ctctaatggc atcgcgaatc ttctgcactg tggcctggtc
      301 tgtctctggt ccctgcagaa agcggccatt gcggcccagg atctcctctc tggaatactc
      361 ggtcagctcc aggaagccat cggatgcgaa gatgatggga ttgtctggca gccgtggatc
      421 agttatcacg aaacttttct ccatggtggc gaccggtgga tccccttcaa ggtcatcgtg
      481 atgctgagaa gtttcgttga taacctgtcc atctctagtt tcaaccgtct taatcagaag
      541 tgtccttttt gagtgggtat caaccagagg gagtgaatcc agattagttt ccctcaggtt
      601 cagggaggaa aagtttggaa gaggcagaga aatcctgctc tcctcgcctt ccagcagctt
      661 cctgtaggtg gcaatctcaa tgtcaagggc catcttaaca ttgagcaggt cttggtattc
      721 acgaaggtga cgagccattt cctccttcat attctgaatc tcatcctgca ggcggccaat
      781 agtgt
//
