LOCUS       sequence-118219-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hSar1 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-SAR1
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(701..725)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(757..776)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(758..776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(821..872)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(850..870)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctatcag tcaatatact gggagagcca gcggaaaccc
      121 tcgccgtaac cttgcctctt gagcacactg cacatgaaca cttccatggg gcgagcattc
      181 agctccttca gggtcacatt cccctttcct gtggtctgtc cataaagccc aaatatctca
      241 cggagttttt cttcactgat tgcatctgtt ctgtcaattt tgttacccaa gataaggatt
      301 ggcacattgg atattgtttc atcagtcatt aaagcattaa gctcaacttt ggattccacg
      361 aggcgagaat gatctgcaca gtccaccaga aagacaatcc cattaattgc tgggagataa
      421 tttttccaaa cgcgacgtgc ttgctcgtgc ccaccaagat caaaagttgt aaaggtcatt
      481 ccagcaattg ttagctcttc tgatgtcgga tgtagtgttg gaacatgttg gcccaatctg
      541 tcatctttga gcatgtgaag aagagtggtt ttgcctgcat tatccaaacc taagaataca
      601 agttttccag atttcttgta cagtcctagg aactggagca cactgctgaa gccattgtag
      661 atccactcaa agatgaaaga catggttcta tctccttcta agcctgcttt tttgtacaaa
      721 cttgtttgat caagcttaac tagccagctt gggtctccct atagtgagtc gtattaattt
      781 cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct
      841 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagt
//
