LOCUS       sequence-118222-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hSara with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-SARA
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(701..725)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(757..776)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(758..776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(821..872)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(850..870)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctattaa tcaatgtact gtgccatcca gcggaagcct
      121 tctccgtaac cttgtctttt gagcacacta cacatgaaaa cttctaaggg tcgggcattc
      181 agttctttca gagatatact cccctttcct gttgtctgac catataaacc aaacatctct
      241 cgcaacctct cttcactgat ggcttcaggt ctgtcgatct tattcccaag aatcagtata
      301 ggcacattag caatggtttc atctgtcatt agtgaatcaa gttcttcttt tgactctaac
      361 agcctttcgt ggtctgcaca atccaccaga aatacaatgc cattgatagc aggaaggtag
      421 tttttccaca ctcttcgagc ttgaacatgt ccacccagat caaaagttgt aaacgtcatg
      481 ccagcaatgg tcagttcttc ggaagtggga tgtaatgttg ggacatgttg tccaagtctg
      541 tcatctttta gcatgtgtag caatgttgtt tttcctgcat tatccaatcc aagaaatacc
      601 agtttaccag ttttcttata taatcctaaa aactgtagca cactgctgaa accactgtaa
      661 atccaatcaa atatgaagga catggttcta tctccttcta agcctgcttt tttgtacaaa
      721 cttgtttgat caagcttaac tagccagctt gggtctccct atagtgagtc gtattaattt
      781 cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct
      841 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
      901 gaaagtcccg ttga
//
