LOCUS       sequence-118224-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic 
            configuration with optimized promoters (E12).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-E12-vioABECD
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..57)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     53..71
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(110..154)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tttcgctagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctagtta
       61 ttgctcagcg gtggcagcag tttaggttaa ttaagctgcg actagtagac gagtccatgt
      121 gctggcgttc aaatttcgca gcagcggttt ctttaccaga ctcgagttaa cgttgcagcg
      181 cataacctaa tgcctttgct agcgcttctg gtaaagaagg cagctgctca cgacgttcag
      241 taaaactttg agcgatttga gaagtactta gatgcattag ctctcctaca ctttcaaacc
      301 aaaggcgact tgtttgtgca tgttgatcaa acaatttcat gacaggcatc actcgcgtat
      361 taaagtgctc taccacagca atgacgttat ttgcatgttg atggacagat ttaatcagta
      421 actgtgcgcc aaccactgcc atggtcgtgc catgcccgat tgaaaaatga cccgattgca
      481 acgcatcgcc caataacaca atatttccat cataggcctt ttcatgactt aacgtcatga
      541 aattacgcca tttcaatcct gattgagcaa taacaggctt tccgttcaaa tcagattcaa
      601 atacggatgc aataaacgct tctgcttggt ctgcagacat ttcgtctaga cctgacttta
      661 gatacgtctc ttcgctacat tcaacaacaa aagtactcat gtcactcgaa tatttatagg
      721 catgagcgac aaaaacacct ttagaatgcg ctttaaaaat caggttcatt tcattaaaaa
      781 gattaggcgt gccataccac atgtagcgat tcgtgccata
//
