LOCUS       sequence-118227-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes accA, accB, accC, accD in Pseudo-operon configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pETM6-P.accABCD
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(18..65)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     61..79
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(118..162)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 actcctcctt tcgctagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat
       61 gctagttatt gctcagcggt ggcagcagtt taggttaatt aagctgcgac tagtagacga
      121 gtccatgtgc tggcgttcaa atttcgcagc agcggtttct ttaccagact cgagtcaggc
      181 ctcaggttcc tgatccggta ccgggggtac cactacgcct tcacgcggcg cttcaggatt
      241 cggcgctggc agattcatca acttcgccag aatgctcgcc agtttcaggc gcatttccgg
      301 acgacggacg atcatgtcga tcgcgccttt ctcgatcagg aattcactgc gctggaatcc
      361 aggcggcagt ttttcgcgaa cggtctgttc gataacacgc ggaccggcaa agccgattaa
      421 cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg aaacttgcag aaacaccgcc
      481 catcgtcggg tcggtcagca cggagatgta cggcaagccg cgctcctgca tttttgccag
      541 tgccgcagag gttttcgcca tctgcatcag cgacatcagt gcttcctgca tacgtgcgcc
      601 accagaggcg gagaagcaga tcagcgggca gttatcttcc agcgcctgct caacggcacg
      661 cacgaaacgt gcacccacaa cagaccccat tgaaccgccc ataaaggcga actcgaatgc
      721 cgcagcgaca accggcattc catacagagt gcctttcatc accaccagcg catctttttc
      781 gccggtttct ttctgcgcag atgccagacg gtctttatac ttcttggagt cacgaaactt
      841 cagcacatct ttcggctcaa gctcgctacc
//
