LOCUS       sequence-118230-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: pLenti-EF1a-SOX2
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..83)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(105..254)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(279..299)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcact gggccaggat tctcctcgac gtcaccgcat
       61 gttagcagac ttcctctgcc ctctccactg cctctagagt taatcagcat gtccaggtcg
      121 aaatcatcaa gggcgtcact gccgagcatg tcgaggtcaa agtcatcaag ggcatccgaa
      181 ccaagcatgt caaggtcaaa atcatcgagg gcgtcacttc ccagcatatc cagatcaaaa
      241 tcgtccaatg cgtcagcccg tccggaaccg ctggcctcca cctttctctt cttcttgggg
      301 ctagctgacg cgcgagtttg gtccccttca tgcatgggcg agggcgcgtc caaatccctc
      361 tccagcgagt ctgcgaacgc atgaaggctc gcctggtcag gtgtctgagt tgacgtaggt
      421 gaaggcttcg cgcgtttcat accgctcgct tggagaatcc tgtcccaccg ctgggaggcg
      481 ggaggcagtg tgcccgagcg ggcctcgagt tctgtgacac cgactctacg aaagagctgc
      541 agcagtccgt gtctcgacat accaaattga gtcatggcgt catcgaacgc ttgtgcgggg
      601 tgggagtgac actggaagaa tccgagcacg cggaccactt gcgcgtgatc tgccactcga
      661 tgtgatgtcc tctcgggaat ccttctgttg gtccgcttaa tcaatgcagg cgcgtgcggg
      721 agccccttct tcaccgcatc caaggcgggt cgtccgccga ggcaggccag cgctacaaga
      781 tgatcattag tgagtgcggc cagcgcgggg tccggcctcg aaagctgggc cacgattgac
      841 tccagtgcgg gtctgccccc gatattggat gcaatagcca ctacctgctc aggcgtgagt
      901 ccgtgcgctt ggcacagcac aggcaacagc ctctgtactg tttccaaggc ttgctttcct
      961 ccgtcgtggc ttgcgatcgc cacgacctgc tctggg
//
