LOCUS       sequence-118231-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl2 with N-term His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST17-6xHis-ARL2
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(670..694)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(747..765)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      761..808
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttgtttact ttaagaagga gatatacata tgtcgtacta ccatcaccat caccatcacc
       61 tcgaatcaac aagtttgtac aaaaaagcag gcttagaagg agatagaacc atggggctcc
      121 tgaccattct gaagaagatg aagcagaaag agcgggagct gcgactgctc atgcttggcc
      181 tggacaatgc tggaaagaca accatcctga agaagttcaa tggggaggac atcgacacca
      241 tctccccaac gctgggcttc aacatcaaga ccctggagca ccgaggattc aagctgaaca
      301 tctgggatgt gggtggccag aagtccctgc ggtcctactg gcggaactac tttgagagca
      361 ccgatggcct catctgggta gtggacagcg cagaccgcca gcgcatgcag gactgccagc
      421 gggagctcca gagcctgctg gtggaggagc gcctggccgg agcaaccctc ctcatctttg
      481 ctaataagca ggacctgcct ggagcactgt cctctaacgc catccgcgag gccctggagc
      541 tggactccat ccgcagccac cactggtgca tccagggctg cagcgccgtc accggggaga
      601 acctgctgcc gggcatcgac tggctcctgg atgacatttc cagccgcatt ttcacagctg
      661 actgatagga cccagctttc ttgtacaaag tggttgattc gaggctgcta acaaagcccg
      721 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      781 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca
      841 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
      901 ggactgggcg
//
