LOCUS       sequence-118233-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein 
            Scaffold GBD:SH3:PDZ (1:1:4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p168-759
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             269..313
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(352..370)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      366..413
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     447..466
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        447..465
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    466..490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             505..527
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 accgattacc tttgccgata tgtataaacg caaaatgagc aaagatatcg agctggccaa
       61 actgaaaaaa ctggcgaaag aaaaaaaact gctgcaagac cagcaggata tcgaaaaagc
      121 aaaactggaa atcaaaagca ccgatgaaat cttcgccctg gttggtgcac tgatgcatgt
      181 tatgcagaaa cgtagccgtg caattcatag cagtgatgaa ggtgaagatc aagccggtga
      241 tgaagatgag gattaactcg agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca
      301 gcacatggac tcgtctacta gtcgcagctt aattaaccta aactgctgcc accgctgagc
      361 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctaggat
      421 cgagatcgat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      481 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat acatatgggt
      541 ctgggtgcag aaagcggtag cgtttgtgtt accggtgcaa gcggttttgt tggtagctgg
      601 ctggttatgc gtctgctgga acatggttat accgttcgtg caaccgtgcg tgatccggca
      661 aatctgaaaa aagttcgtca tctgctggaa ctgccgcagg cagcaacccg tctgaccctg
      721 tggaaagcag atctggatgt tgaaggtagc tttgatgaag ccattaaagg ttgtaccggt
      781 gtttttcatg ttgcaacccc gatggatttt gaaagcgaag atccggaaaa cgaagttatt
      841 aaaccgacca ttaacggcat gctggatatt atgaaagcat gcctgaaagc aaa
//
