LOCUS       sequence-118236-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein 
            Scaffold GBD:SH3:PDZ (1:1:4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p168-759
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             237..259
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    274..298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(298..317)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(299..317)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(351..398)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     394..412
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(451..495)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tgcattcttt atcgttgatc acgccataaa tcactttggc acctttatcc tgaaaggttt
       61 tgataataac tgctttgctc gggctaaccg gacccggacg aaccagcaga aaggtcggat
      121 aaccaaaacc caggcttgct ttggtaataa actgaccaat aaaaccggtg ccaccaacaa
      181 ccagggtacg atttttggtc atgctcggaa ttgcaccgct aacggtcata tgtatatctc
      241 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
      301 tatagtgagt cgtattaatt tcgcgggatc gagatcgatc tcgatcctag caaaaaaccc
      361 ctcaagaccc gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca
      421 gtttaggtta attaagctgc gactagtaga cgagtccatg tgctggcgtt caaatttcgc
      481 agcagcggtt tctttaccag actcgagtta acgacgacgt ttcggaggca gtgcaggagg
      541 aggacctgca cgacgttttt cggtttcttc ttcttgcggc agcggcagca gacctttttc
      601 acgacatgca tcaacggcac ctgtaaacat atcttccagg ctgtgtttaa attcgaagcc
      661 catctctttc agttttttgc tgctaaagtg aatgttggtc aggttttctt cgatgccttt
      721 gaattttttc ggaacgttgt atttcgggta tttctcgttc agcagttttg caatatcgtg
      781 aatggttgca tcatggctgc tacaaatata acgacc
//
