LOCUS       sequence-118238-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p158
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             236..258
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    273..297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(297..316)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(298..316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(350..397)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     393..411
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(450..494)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gcattcttta tcgttgatca cgccataaat cactttggca cctttatcct gaaaggtttt
       61 gataataact gctttgctcg ggctaaccgg acccggacga accagcagaa aggtcggata
      121 accaaaaccc aggcttgctt tggtaataaa ctgaccaata aaaccggtgc caccaacaac
      181 cagggtacga tttttggtca tgctcggaat tgcaccgcta acggtcatat gtatatctcc
      241 ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct
      301 atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctagc aaaaaacccc
      361 tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag
      421 tttaggttaa ttaagctgcg actagtagac gagtccatgt gctggcgttc aaatttcgca
      481 gcagcggttt ctttaccaga ctcgagttaa cgacgacgtt tcggaggcag tgcaggagga
      541 ggcactttgt tgccattaac cggattttct gcaaagctat gcggcagcag acctttttca
      601 cgacaggtat caatggcacc acgaaacata tcttccaggc tatatttgaa ctcgaagccc
      661 atgccaatca gttttttgct ggaaaagcta acaaccggca gatctttatc gatgcctttg
      721 aattcggtcg gaacattata ttcaggccat ttttcacgca tcagtttggc cagatcatga
      781 atggttgcat catggctgct acaaatataa cgaccttctg
//
