LOCUS       sequence-118241-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p158
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             265..309
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(348..366)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      362..409
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     443..462
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        443..461
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    462..486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             501..523
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 attacctttg ccgatatgta taaacgcaaa atgagcaaag atatcgagct ggccaaactg
       61 aaaaaactgg cgaaagaaaa aaaactgctg caagaccagc aggatatcga aaaagcaaaa
      121 ctggaaatca aaagcaccga tgaaatcttc gccctggttg gtgcactgat gcatgttatg
      181 cagaaacgta gccgtgcaat tcatagcagt gatgaaggtg aagatcaagc cggtgatgaa
      241 gatgaggatt aactcgagtc tggtaaagaa accgctgctg cgaaatttga acgccagcac
      301 atggactcgt ctactagtcg cagcttaatt aacctaaact gctgccaccg ctgagcaata
      361 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc taggatcgag
      421 atcgatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
      481 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat atgaaagata
      541 gcgttgcaag cgcaaccgca agcgcaccgg gtacagtttg tgttaccggt gcagcaggtt
      601 ttattggtag ctggctggtt atgcgtctgc tggaacgtgg ttatattgtt cgtgcaaccg
      661 ttcgtgatcc ggcaaatctg aaaaaagtta aacatctgct ggatctgccg aaagcagata
      721 ccaatctgac cctgtggaaa gccgatctga atgaagaggg tagctttgat gaagcaattg
      781 aaggttgtag cggtgttttt catgttgca
//
