LOCUS       sequence-118242-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AS-30D hepatoma hexokinase II 4.3 kbp promoter-luciferase reporter 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Mathupala SP, Rempel A, Pedersen PL
  TITLE     Glucose catabolism in cancer cells: identification and 
            characterization of a marked activation response of the type II 
            hexokinase gene to hypoxic conditions.
  JOURNAL   J Biol Chem. 2001 Nov 16;276(46):43407-12. Epub 2001 Sep 13.
  PUBMED    11557773
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-11-16"; volume: "276(46)"; pages: "43407-12. 
            Epub 2001 Sep 13"
COMMENT     Sequence Label: pGL2-HKII-A
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    154..275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(191..210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     245..264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     352..371
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        352..370
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggcgatcggt gcgggcctct tcgctattac gccagcccaa gctaccatga taagtaagta
       61 atattaaggt acgtggaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
      121 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
      181 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      241 agttgtggtt tgtccaaact catcaatgta tcttatggta ctgtaactga gctaacataa
      301 cccgggaggt accgagctct tacgcgtgct agagctcgcg gccgcgagct ctaatacgac
      361 tcactatagg gcgtcgactc gatccaactg gcctagaact cacagccatc ctcttgcctc
      421 tacctatgga gtgtggagat taaaggcatg ttctaccatg tcttaatttt aaaataccta
      481 tggagtgtgg agattaaagg catgttctac catgtcttaa ttttaaaata ggaatatttg
      541 tggattgagg tcttgagcaa aataagattt ttcccaagag agtttcctga agcctaagta
      601 gactcaggtc cttctcatgc agggccaatc tagggccagg agcaggacca actggtgtga
      661 aatcagaaag atggtactca tagctattag tccatctctg gttgacactc ccagactccc
      721 ctacatctca agacacagac atacgtggct tttatgaatc catttttctg gtctgtatta
      781 tttgttctgt gtgttaattt tatgtcttaa actaaacagt aatcctttaa ggaaagaaca
      841 ccccgcccct ctcctgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgcacg tctgtgtgag
      901 tatcttgcac cctgtaaagg gcttaataaa cacgtgctga ttgattttcc tcctggaatg
      961 tgaatgtgga ctgccattct gccagtcttc aatgtttg
//
