LOCUS       sequence-118244-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes accA, accB, accC, accD in monocistronic configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pETM6-M.accABCD
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             276..298
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    313..337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(337..356)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(338..356)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(390..437)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     433..451
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(490..534)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg gtgatttcag
       61 cggcgctgat gattgccggg atgttcagat aactttttac tgacggagca gggccaatac
      121 agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg gagtgcacag
      181 cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca atctcgccgc
      241 ggttggcaat aacaatttta tccagcatat gtatatctcc ttcttaaagt taaacaaaat
      301 tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt
      361 cgcgggatcg agatcgatct cgatcctagc aaaaaacccc tcaagacccg tttagaggcc
      421 ccaaggggtt atgctagtta ttgctcagcg gtggcagcag tttaggttaa ttaagctgcg
      481 actagtagac gagtccatgt gctggcgttc aaatttcgca gcagcggttt ctttaccaga
      541 ctcgagttac tcgatgacga ccagcggctc gtcaaattct accggttgtc cactttcgac
      601 cagaattgct ttcacggtac cggatttgtc cgcttcgatc tggttcatca ttttcatggc
      661 ttcaacgatg cacagggtat cgcccacgtt gactttctga cccacttcga tgaacgcttt
      721 tgcgtccggg cttggggtgc ggtagaaagt accaaccatc ggggaacgta cgatgtgacc
      781 actgatttcc gctgctgctg
//
