LOCUS       sequence-118247-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p168
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             266..310
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(349..367)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      363..410
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     444..463
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        444..462
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    463..487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             502..524
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gattaccttt gccgatatgt ataaacgcaa aatgagcaaa gatatcgagc tggccaaact
       61 gaaaaaactg gcgaaagaaa aaaaactgct gcaagaccag caggatatcg aaaaagcaaa
      121 actggaaatc aaaagcaccg atgaaatctt cgccctggtt ggtgcactga tgcatgttat
      181 gcagaaacgt agccgtgcaa ttcatagcag tgatgaaggt gaagatcaag ccggtgatga
      241 agatgaggat taactcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca
      301 catggactcg tctactagtc gcagcttaat taacctaaac tgctgccacc gctgagcaat
      361 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctaggatcga
      421 gatcgatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
      481 caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgggtctg
      541 ggtgcagaaa gcggtagcgt ttgtgttacc ggtgcaagcg gttttgttgg tagctggctg
      601 gttatgcgtc tgctggaaca tggttatacc gttcgtgcaa ccgtgcgtga tccggcaaat
      661 ctgaaaaaag ttcgtcatct gctggaactg ccgcaggcag caacccgtct gaccctgtgg
      721 aaagcagatc tggatgttga aggtagcttt gatgaagcca ttaaaggttg taccggtgtt
      781 tttcatgttg caaccccgat
//
