LOCUS       sequence-118250-        1111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the wild type of CYP119 fused to Sulfolobus solfataricus 
            PCNA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Suzuki R, Hirakawa H, Nagamune T
  TITLE     Electron donation to an archaeal cytochrome P450 is enhanced by 
            PCNA-mediated selective complex formation with foreign redox 
            proteins.
  JOURNAL   Biotechnol J. 2014 Dec;9(12):1573-81. doi: 10.1002/biot.201400007. 
            Epub 2014 Jul 21.
  PUBMED    24924478
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201400007"; journalName: "Biotechnol J"; date: 
            "2014-12"; volume: "9"; issue: "12"; pages: "1573-81"
COMMENT     Sequence Label: pET11+PCNA3-CYP119
FEATURES             Location/Qualifiers
     source          1..1111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgatctacct gaaatctttc gaacgtaaca tccgcctgat
      181 caatatgaaa gtggtttacg atgatgttcg tgtgctgaaa gatattatcc aggcgctggc
      241 ccgcctggtt gatgaagccg tgctgaaatt caaacaggat agcgttgaac tggtggcact
      301 ggatcgtgcg catattagcc tgatctctgt taacctgccg cgcgaaatgt tcaaagaata
      361 cgatgtgaac gatgaattta aattcggttt caacacccag tacctgatga aaatcctgaa
      421 agttgcaaaa cgtaaagaag ccattgaaat cgcaagtgaa agcccggata gtgtgattat
      481 caacattatc ggcagcacca atcgtgaatt taacgttcgc aatctggaag tgtctgaaca
      541 ggaaattccg gaaatcaacc tgcagttcga tattagtgcg acgatcagct ctgatggttt
      601 taaaagtgcc atcagcgaag tttctaccgt gacggataat gtggttgtgg aaggtcacga
      661 agatcgcatt ctgatcaaag cagaaggcga aagcgaagtt gaagtggaat tttctaaaga
      721 taccggcggt ctgcaggatc tggaatttag taaagaaagc aaaaattctt acagtgcgga
      781 atacctggat gatgttctgt ctctgacgaa actgagtgat tacgtgaaaa tcagcttcgg
      841 caaccagaaa ccgctgcagc tgtttttcaa tatggaaggc ggtggcaaag ttacgtacct
      901 gctggcgccg aaagtgggcg gtagcatgac gactgaaacc atacaaagca acgccatgta
      961 tgactggttt agtgagatga gaaagaaaga cccagtgtat tatgacggaa acatttggca
     1021 ggtgttttcc tataggtaca caaaggaggt tttaaacaac ttttcgaaat tctcctcaga
     1081 tcttacaggt taccacgaaa ggcttgagga c
//
