LOCUS       sequence-118252-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p148
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             233..255
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    270..294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(294..313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(295..313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(347..394)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     390..408
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(447..491)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ttctttatcg ttgatcacgc cataaatcac tttggcacct ttatcctgaa aggttttgat
       61 aataactgct ttgctcgggc taaccggacc cggacgaacc agcagaaagg tcggataacc
      121 aaaacccagg cttgctttgg taataaactg accaataaaa ccggtgccac caacaaccag
      181 ggtacgattt ttggtcatgc tcggaattgc accgctaacg gtcatatgta tatctccttc
      241 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
      301 gtgagtcgta ttaatttcgc gggatcgaga tcgatctcga tcctagcaaa aaacccctca
      361 agacccgttt agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagttt
      421 aggttaatta agctgcgact agtagacgag tccatgtgct ggcgttcaaa tttcgcagca
      481 gcggtttctt taccagactc gagttaacga cgacgtttcg gaggcagtgc aggaggagga
      541 tgaccattat cctgaccggt tgcaatcagc tgttcggttg catagctcgg ttctttggtt
      601 gccggaggca gcagaccttt ttcacgacag ctctgaattg caccatcgaa catatcttcc
      661 aggctatatt tgaattcgaa acccagatcc gtcagttttt tgctgctata gctgatgata
      721 tcaaacactt ccatttcacc aaattcggtc ggaacatcaa attccggata acgctgacgc
      781 agaatctgtg ccagacctgc aatggtaaca tcatggctgc t
//
