LOCUS       sequence-118255-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes accA, accB, accC, accD in Pseudo-operon configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pETM6-P.accABCD
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             219..241
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    256..280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(280..299)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(281..299)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(337..381)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 cagcggcgct gatgattgcc gggatgttca gataactttt tactgacgga gcagggccaa
       61 tacagaccgt ttcatctgcc agtaatangt gttttagatc gcgatccgcg ctggagtgca
      121 cagcgacagt cttgatgccc agttctttac aggcacgaag aatacgcaat gcaatctcgc
      181 cgcggttggc aataacaatt ttatccagca tatgtatatc tccttcttaa agttaaacaa
      241 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      301 tttcgcggga tcgagatcga tctcgatcct agtagacgag tccatgtgct ggcgttcaaa
      361 tttcgcagca gcggtttctt taccagactc gagttactcg atgacgacca gcggctcgtc
      421 aaattctacc ggttgtccac tttcgaccag aattgctttc acggtaccgg atttgtccgc
      481 ttcgatctgg ttcatcattt tcatggcttc aacgatgcac agggtatcgc ccacgttgac
      541 tttctgaccc acttcgatga acgcttttgc gtccgggctt ggggtgcggt agaaagtacc
      601 aaccatcggg gaacgtacga tgtgaccact gatttccgct gctgctggcg cttccatgga
      661 aggaacggtc gccggagcgg ctgcgttaga ttgagctggc tgctgcatca ttggtgcagc
//
