LOCUS       sequence-118261-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic 
            configuration with G6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-G6-vioABECD
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..129
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(168..186)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      182..229
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    282..306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             321..343
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tttcctaatt ttaatacgct gccgatacct aacacgattt ttgatgtaac aagtctctct
       61 cataggaatt gactcgagtc tggtaaagaa accgctgctg cgaaatttga acgccagcac
      121 atggactcgt ctactagtcg cagcttaatt aacctaaact gctgccaccg ctgagcaata
      181 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc taggatcgag
      241 atcgatctcg atcccgcgaa attaatacga ctcactattt cggaattgtg agcggataac
      301 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat atgagtaaaa
      361 taattattgt tggtggtggg ttggcaggca gcctgacggc aatctatcta gcgaaacaag
      421 ggcacgaagt tcacattata gaaaaacgtg gaaatccttt agaaagtcag gcggattata
      481 ttgatcaagt tagcgcgcgt gcgattggtg tcagtatgac agtacgagga attgaagccg
      541 ttttagcagc cggtattccc ttggacgaat tgcaggcgtg cggcgttgaa gtttcaggta
      601 tggcgttttt tatcaaaggt caacctaaaa tacgggattt accaccgtta gatacgttaa
      661 aaccgttgtc gttaagccga agcatgtttc aggtattgct taacaagtac gctcataaag
      721 caaaagtaca ctatcactat ctacaacgct gtgtcgacgt tgatattgaa agagcgtcag
      781 t
//
