LOCUS       sequence-118266-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP 
            expression cassette - Kanamycin plasmid 
            [A:J23102:B:BCD2:C:E0040m:D:B0015:E].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Gm_AE
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..23
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(98..132)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     misc_feature    152..173
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      176..219
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(223..241)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(435..454)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(519..538)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cgcatcatta gaaaacctcc ttagcatgat taagatgttt cagtacgaaa attgctttca
       61 ttgttgatct cctttttaag tgaacttggg cccagtagct agcacagtac ctaggactga
      121 gctagctgtc aactccatgt cttccactag tctctagaag cggccgcgaa ttccagaaat
      181 catccttagc gaaagctaag gatttttttt atctgaaatt ctgcctcgtg atacgcctat
      241 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaagtggc actcgagctg
      301 atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct
      361 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
      421 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctccag
      481 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
      541 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
      601 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
      661 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
      721 tgatgcatgg ttactcacca ctgcgatccc cgggaaa
//
