LOCUS       sequence-118268-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic 
            configuration with optimized promoters (E12).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-E12-vioABECD
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             133..177
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(216..234)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      230..277
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    331..354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             369..391
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctccgaatgg tcacgggtga tccgaacgtt cacgcctctg taagagactt tcctaatttt
       61 aatacgctgc cgatacctaa cacgattttt gatgtaacaa gtctctctca tagaaattga
      121 ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat ggactcgtct
      181 actagtcgca gcttaattaa cctaaactgc tgccaccgct gagcaataac tagcataacc
      241 ccttggggcc tctaaacggg tcttgagggg ttttttgcta ggatcgagat cgatctcgat
      301 cccgcgaaat taatacgact cactacggaa gaattgtgag cggataacaa ttcccctcta
      361 gaaataattt tgtttaactt taagaaggag atatacatat gagtaaaata attattgttg
      421 gtggtgggtt ggcaggcagc ctgacggcaa tctatctagc gaaacaaggg cacgaagttc
      481 acattataga aaaacgtgga aatcctttag aaagtcaggc ggattatatt gatcaagtta
      541 gcgcgcgtgc gattggtgtc agtatgacag tacgaggaat tgaagccgtt ttagcagccg
      601 gtattccctt ggacgaattg caggcgtgcg gcgttgaagt ttcaggtatg gcgtttttta
      661 tcaaaggtca acctaaaata cgggatttac caccgttaga tacgttaaaa ccgttgtcgt
      721 taagccgaag catgtttcag gtattgctta acaagtacgc tcataaagca aaagtacact
      781 atcactatct acaacgctgt gtcgacgttg atattgaaag agcgtcagtg atta
//
