LOCUS       sequence-118272-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is an envelope gene encoding plasmid to make retrogradely 
            lentiviral vector. You can make type B envelope coating virus 
            particle with HiRet by using this plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Kato S, Kobayashi K, Inoue K, Kuramochi M, Okada T, Yaginuma H, 
            Morimoto K, Shimada T, Takada M, Kobayashi K
  TITLE     A lentiviral strategy for highly efficient retrograde gene transfer 
            by pseudotyping with fusion envelope glycoprotein.
  JOURNAL   Hum Gene Ther. 2011 Feb;22(2):197-206. doi: 10.1089/hum.2009.179. 
            Epub 2011 Jan 27.
  PUBMED    20954846
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/hum.2009.179"; journalName: "Hum Gene Ther"; date: 
            "2011-02"; volume: "22"; issue: "2"; pages: "197-206"
COMMENT     Sequence Label: pCAGGS-FuG-B
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     315..334
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    333..388
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     434..453
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(627..659)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 ggtcgaggga tctccataag agaagaggga cagctatgac tgggagtagt caggagagga
       61 ggaaaaatct ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata
      121 acacaaaaca aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta tggaataagg
      181 aatggacagc agggggctgt ttcatatact gatgacctct ttatagccac ctttgttcat
      241 ggcagccagc atatggcata tgttgccaaa ctctaaacca aatactcatt ctgatgtttt
      301 aaatgatttg ccctcccata tgtccttccg agtgagagac acaaaaaatt ccaacacact
      361 attgcaatga aaataaattt cctttattag ccagaagtca gatgctcaag gggcttcatg
      421 atgtccccat aatttttggc agagggaaaa agatctgcta gctatagttc tagaggtacc
      481 ggttgttggg ggctgcagga attccgtttt tttttttttt tttcataaaa attaaaaact
      541 caaatataat tgaggcctct ttgagcatgg tatcacaagt tgatttggtc caaacatgaa
      601 gaatctgttg tgcaggattt gagttacttt ccaagtcggt tcatctctat gtctgtataa
      661 atctgtcttt tcttggtgtg ctttaattta atgcaaagat ggataccaac tcttctgcac
      721 catgtcatta gggaaaatat caacaccagg ccaatcatgg cccctgc
//
