LOCUS       sequence-118273-         510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic 
            configuration with consensus promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-vioABECD
FEATURES             Location/Qualifiers
     source          1..510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..118
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(157..175)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      171..218
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     252..271
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        252..270
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    271..295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             310..332
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 taatacgctg ccgataccta acacgatttt tgatgtaaca agtctctctc ataggaattg
       61 actcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc
      121 tactagtcgc agcttaatta acctaaactg ctgccaccgc tgagcaataa ctagcataac
      181 cccttggggc ctctaaacgg gtcttgaggg gttttttgct aggatcgaga tcgatctcga
      241 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
      301 agaaataatt ttgtttaact ttaagaagga gatatacata tgagtaaaat aattattgtt
      361 ggtggtgggt tggcaggcag cctgacggca atctatctag cgaaacaagg gcacgaagtt
      421 cacattatag aaaaacgtgg aaatccttta gaaagtcagg cggattatat tgatcaagtt
      481 agcgcgcgtg cgattggtgt cagtatgaca
//
