LOCUS       sequence-118276-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFPuv Expression, Low Phosphate Dependent E. coli Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, 
            Wang R, Lynch MD
  TITLE     Scalable, two-stage, autoinduction of recombinant protein expression
            in E. coli utilizing phosphate depletion.
  JOURNAL   Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440.
  PUBMED    32441815
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2020 May 22. doi: 10.1002/bit.27440."
COMMENT     Sequence Label: pSMART-GFPuv
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(60..162)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(163..194)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(424..444)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcatt
       61 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
      121 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caagtcaaaa gcctccggtc
      181 ggaggctttt gactttctgc tatggaggtc aggtatgatt taaatggtca gtattgagcg
      241 atatctagag aattcgtctt tcttttgggt atagcgtcgt ggacagtcat tcatctttct
      301 gcccctccaa aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg tgaaactgac
      361 cgataagccg gggatcctca ttatttgtag agctcatcca tgccatgtgt aatcccagca
      421 gcagttacaa actcaagaag gaccatgtgg tcacgctttt cgttgggatc tttcgaaagg
      481 gcagattgtg tcgacaggta atggttgtct ggtaaaagga cagggccatc gccaattgga
      541 gtattttgtt gataatggtc tgctagttga acggatccat cttcaatgtt gtggcgaatt
      601 ttgaagttag ctttgattcc attcttttgt ttgtctgccg tgatgtatac attgtgtgag
      661 ttatagttgt actcgagttt gtgtccgaga atgtttccat cttctttaaa atcaatacct
      721 tttaactcga tacgattaac aagggtatca ccttcaaact tgacttcagc acgcgtcttg
      781 tagttcccgt catctttgaa agatatagtg cgttcctgta cataaccttc gggcatggca
      841 ctcttgaaaa agtcatgccg tttcatatga tccggataac gggaaaagca ttgaacacca
      901 taagagaaag tagtgacaag tgttggccat ggaacaggta gttttccagt agtgcaaata
      961 aatttaaggg taagctttcc gtatgtagca tcaccttcac cctctccact gacagaaaat
     1021 ttgtgcccat taacatcacc atctaattca acaagaattg gg
//
