LOCUS       sequence-118280-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for amiR-eGFP knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pAWK
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(306..326)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      329..338
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(379..398)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(460..482)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 aattcccaag caggaattaa tttctttaat gaggcttcca gaattgttgc tttttgcgtc
       61 ttgtatttaa actggagtga tttgttgaca atatcgaaac tcagcgaatt gcttatgata
      121 gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgagt aatcatccaa
      181 cataaatagg ttagttcagc agcacatgat gctatttttt cccctgaagg tctttcaaac
      241 ctttccacaa actgacgaac caggacctta ggtggtgttt tacataatat atcaaattga
      301 ctcatcacct tcctcttctt cttgggagcc atggtggcga ccggtggatc ccaattcttt
      361 gccaaaatga tgagacagca caataaccag cacgttgccc aggagctgta ggaaaaagaa
      421 gaaggcatga acatggttag cagaggctct agagccgccg gtcacacgcc agaagccgaa
      481 ccccgccctg ccccgtcccc cccgaaggca gccgtccccc cgcggacagc cccgaggctg
      541 gagagggaga aggggacggc ggcgcggcga cgcacgaagg ccctccccgc ccatttcctt
      601 cctgccggcg ccgcaccgct tcgccccgcg cccgctagag ggggtgcggc ggcgcctccc
      661 agatttcggc tccgcacaga tttgggacaa aggaagtccc tgcgccctct cgcacgatta
      721 ccataaa
//
