LOCUS       sequence-118282-        9825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for amiR-eGFP knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 9825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pAWK
FEATURES             Location/Qualifiers
     source          1..9825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(59..78)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     288..305
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    492..525
                     /label=loxJTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    536..569
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     enhancer        584..963
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        966..1241
                     /label=chicken beta-actin promoter
     intron          1242..2250
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     ncRNA           2337..2363
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           2427..2468
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           2475..2501
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           2565..2606
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     polyA_signal    2784..2905
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2929..2945)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2929..2945)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    2960..2993
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    3117..3238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(3376..4647,4648..4668,4669..4674))
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPe
                     /note="FLPe is a fusion between the SV40 nuclear 
                     localization signal and a thermostable version of the 
                     Saccharomyces cerevisiae site-specific recombinase FLP. 
                     Recombination occurs at FRT sequences (Buchholz et al., 
                     1998)."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     regulatory      4671..4680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          4748..5759
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(5760..6035)
                     /label=chicken beta-actin promoter
     enhancer        6038..6341
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             7379..7570
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7564..7586)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7672..7812
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7727..7746
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7827..7844)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7998..8586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8078..8097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8757..9548,9549..9617))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9380..9399
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9618..9722)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9790..9808
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggtac cttctgggca tccttcagcc ccttgttgaa
       61 tacgcttgag gagagccatt tgactctttc cacaactatc caactcacaa cgtggcactg
      121 gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc agaggcacct
      181 gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg tttgtcttca
      241 agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca ttcctttggc
      301 gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt tccgggccct
      361 cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg cacaccggcc
      421 ttattccaag cggcttcggc cagtaacgtt aggggggggg gagggagagg ggcggaattc
      481 ttaattctag cataaattgc tataatgtat gctatacgaa gttattgcct aggagataac
      541 ttcgtataaa gtatcctata cgaagttatt ctagcggccg ctcgacattg attattgact
      601 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      661 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      721 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      781 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      841 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      901 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      961 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     1021 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     1081 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
     1141 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     1201 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
     1261 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     1321 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     1381 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     1441 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1501 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     1561 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1621 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1681 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
     1741 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
     1801 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1861 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1921 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1981 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
     2041 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     2101 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
     2161 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     2221 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg
     2281 tctcatcatt ttggcaaaga attgggatcc accggtagtg agtcgaccag tggatcctgg
     2341 aggcttgctg aaggctgtat gctgatgaac ttcagggtca gcttggtttt ggccactgac
     2401 tgaccaagct gactgaagtt catcaggaca caaggcctgt tactagcact cacatggaac
     2461 aaatggccca gatcctggag gcttgctgaa ggctgtatgc tgtgtagttg tactccagct
     2521 tgtgttttgg ccactgactg acacaagctg gtacaactac acaggacaca aggcctgtta
     2581 ctagcactca catggaacaa atggcccaga tctggccgca ctcgagatat ctagacccag
     2641 ctttcttgta caagtaaagc ggccggccgc gactctagat cataatcagc cataccacat
     2701 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2761 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2821 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2881 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatga tccactagtt
     2941 ctagagcggc cgctctagcg aagttcctat tctctagaaa gtataggaac ttcgtactag
     3001 cagttatacg ccaagctggc gcgcgcgccg cacacaaaaa ccaacacaca gatcatgaaa
     3061 ataaagctct tttattggta cctctagtgg atcattaacg cttacaattt acgccttaag
     3121 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3181 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3241 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     3301 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccggc
     3361 cgctttactt gtacattata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc
     3421 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag
     3481 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc
     3541 ctttgatatt ggatcatatg catagtaccg agaaactagt gcgaagtagt gatcaggtat
     3601 tgctgttatc tgatgagtat acgttgtcct ggccacggca gaagcacgct tatcgctcca
     3661 atttcccaca acattagtca actccgttag gcccttcatt gacagaaatg aggtcatcaa
     3721 atgtcttcca atgtgagatt ttgggccatt ctttatagca aagattggat aaggcgcatt
     3781 tttcttcaaa gccttgttgt acgatctgac taagttatct tttaataatt ggtattcctg
     3841 tttgttgctt gaagaattgc cggtcctatt tactcgtttt aggactggtt cagaattcct
     3901 caaaaattca tccaaatata caagtggatc gatcctaccc cttgcgctaa agaagtatat
     3961 gtgcctacta acgcttgtct ttgtctctgt cactaaacac tggattatta ctcccagata
     4021 cttattttgg actaatttaa atgatttcgg atcaacgttc ttaatatcgc tgaatcttcc
     4081 acaattgatg aaagtagcta ggaagaggaa ttggtataaa gtttttgttt ttgtaaatct
     4141 cgaggtatac tcaaacgaat ttagtatttt ctcagtgatc tcccagatgc tttcaccctc
     4201 acttagaagt gctttaagca tttttttact gtggctattt cccttatctg cttcttccga
     4261 tgattcgaac tgtaattgca aactacttac aatatcagtg atatcagatt gatgtttttg
     4321 tccattgtaa ggaataattg taaattccca agcaggaatt aatttcttta atgaggcttc
     4381 cagaattgtt gctttttgcg tcttgtattt aaactggagt gatttgttga caatatcgaa
     4441 actcagcgaa ttgcttatga tagtattata gctcatgaat gtggctctct tgattgctgt
     4501 tccgttatga gtaatcatcc aacataaata ggttagttca gcagcacatg atgctatttt
     4561 ttcccctgaa ggtctttcaa acctttccac aaactgacga accaggacct taggtggtgt
     4621 tttacataat atatcaaatt gactcatcac cttcctcttc ttcttgggag ccatggtggc
     4681 gaccggtgga tcccaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc
     4741 ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc
     4801 cggtcacacg ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc
     4861 cccgcggaca gccccgaggc tggagaggga gaaggggacg gcggcgcggc gacgcacgaa
     4921 ggccctcccc gcccatttcc ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag
     4981 agggggtgcg gcggcgcctc ccagatttcg gctccgcaca gatttgggac aaaggaagtc
     5041 cctgcgccct ctcgcacgat taccataaaa ggcaatggct gcggctcgcc gcgcctcgac
     5101 agccgccggc gctccggggc cgccgcgccc ctcccccgag ccctccccgg cccgaggcgg
     5161 ccccgccccg cccggcaccc ccacctgccg ccaccccccg cccggcacgg cgagccccgc
     5221 gccacgcccc gcacggagcc ccgcacccga agccgggccg tgctcagcaa ctcggggagg
     5281 ggggtgcagg ggggggttac agcccgaccg ccgcgcccac accccctgct caccccccca
     5341 cgcacacacc ccgcacgcag cctttgttcc cctcgcagcc cccccgcacc gcggggcacc
     5401 gcccccggcc gcgctcccct cgcgcacacg cggagcgcac aaagccccgc gccgcgcccg
     5461 cagcgctcac agccgccggg cagcgcgggc cgcacgcggc gctccccacg cacacacaca
     5521 cgcacgcacc ccccgagccg ctcccccccg cacaaagggc cctcccggag ccctttaagg
     5581 ctttcacgca gccacagaaa agaaacgagc cgtcattaaa ccaagcgcta attacagccc
     5641 ggaggagaag ggccgtcccg cccgctcacc tgtgggagta acgcggtcag tcagagccgg
     5701 ggcgggcggc gcgaggcggc gcggagcggg gcacggggcg aaggcaacgc agcgactccc
     5761 gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga
     5821 aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc
     5881 cgcccctcgc cccgccccgc cccgcctggc gcgcgccccc cccccccccg cccccatcgc
     5941 tgcacaaaat aattaaaaaa taaataaata caaaattggg ggtggggagg ggggggagat
     6001 ggggagagtg aagcagaacg tggggctcac ctcgacccat ggtaatagcg atgactaata
     6061 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     6121 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     6181 gtactgccaa gtgggcagtt taccgtaaat agtccaccca ttgacgtcaa tggaaagtcc
     6241 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     6301 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     6361 tatgaactaa tgaccccgta attgattact attaataact agaggccgac gcgctgggct
     6421 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     6481 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     6541 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg
     6601 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
     6661 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
     6721 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
     6781 aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca
     6841 gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt
     6901 tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
     6961 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
     7021 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
     7081 ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat
     7141 cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca
     7201 ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc
     7261 acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc
     7321 tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt
     7381 gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac
     7441 gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct
     7501 tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga
     7561 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     7621 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     7681 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     7741 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     7801 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7861 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7921 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7981 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8041 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8161 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8401 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     8461 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8521 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8581 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8641 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8701 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8761 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8821 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8881 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8941 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9001 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9061 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9121 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9181 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9241 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9301 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9361 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9421 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9481 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9541 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9601 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9721 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     9781 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaa
//
