LOCUS       sequence-118283-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control guide RNA plasmid E. coli , low phosphate induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     SUPERSEDE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCASCADE
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      68..139
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      155..182
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(513..530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(727..746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct agacatcatc
       61 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      121 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      181 cgtttataca cagctaacac cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt
      241 gctggataac tctttctgac accttactat cttacaaatg taacaaaaaa gttatttttc
      301 tgtaattcga gcatgtcatg ttaccccgcg agcataaaac gcgtatattc agggagacca
      361 caacggtttc cctctacaaa taattttgtt taactttgaa ttcaaaagat ctggtaccac
      421 ctcgagttcc ccgcgccagc ggggataaac cgaaaaaaaa accccgcccc tgacagggcg
      481 gggttttttt tcctagggat atattccgct tcctcgctca ctgactcgct acgctcggtc
      541 gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc
      601 aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc
      661 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
      721 gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg
      781 cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg
      841 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccc
//
