LOCUS       sequence-118285-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photoactivatable myosin light-chain kinase inihibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Yi JJ, Wang H, Vilela M, Danuser G, Hahn KM
  TITLE     Manipulation of endogenous kinase activity in living cells using 
            photoswitchable inhibitory peptides.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):788-95. doi: 10.1021/sb5001356. 
            Epub 2014 Jun 13.
  PUBMED    24905630
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb5001356";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "788-95"
COMMENT     Sequence Label: mVenus-PA-MKI
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(656..907)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(698..719)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttagctgcat aacaggttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      121 gatcagttat ctagatccgg tggatcccgg gcccgcggta ccgtcgactg cagaattcct
      181 atttacgacg atatttatat tttttacgtc cctcatcaat attttctgca gttttcttaa
      241 tcagcatgac tccctctctc tcggcagcat ctcggacatg ctcagttcca tccaactgaa
      301 ccccaataaa gtactggaca tctcccttct gatctcgcat aggctgcaag tgaaagaggt
      361 tccagaactt tttaccactc tttgtataat taatcagctg aacagtgacc tctgtttggt
      421 tatctatggc atctctaatt tttctcactg tcgcgcgatc agtttcagga ccttgtagaa
      481 acctgcagtt tcttcccaaa atttcttcac ggctatattc tgtcaactgc aagaaactat
      541 cggacgcgaa tataatggga ttatctggca atcttgggtc agtaatgaca aagttcttct
      601 caatacgttc aagtgtagta gccaaggatc ccatttctcg agatctgagt ccggacttgt
      661 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
      721 gatcgcgctt ctcgttgggg tctttgctca gtttggactg gtagctcagg tagtggttgt
      781 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      841 gcacgccgcc gtcctcgatg ttgtggcgga tcttgaagtt tgccttgatg ccgttcttct
      901 gcttgtcggc ggtgatatag acgttgtggc tgtt
//
