LOCUS       sequence-118297-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is an envelope gene encoding plasmid to make retrogradely 
            lentiviral vector. You can make type B2 envelope coating virus 
            particle with HiRet by using this plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Kato S, Kuramochi M, Kobayashi K, Fukabori R, Okada K, Uchigashima 
            M, Watanabe M, Tsutsui Y, Kobayashi K
  TITLE     Selective neural pathway targeting reveals key roles of 
            thalamostriatal projection in the control of visual discrimination.
  JOURNAL   J Neurosci. 2011 Nov 23;31(47):17169-79. doi: 
            10.1523/JNEUROSCI.4005-11.2011.
  PUBMED    22114284
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.4005-11"; journalName: "J Neurosci"; date: 
            "2011-11-23- 23"; volume: "31"; issue: "47"; pages: "17169-79"
COMMENT     Sequence Label: pCAGGS-FuG-B2
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     311..330
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    329..384
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     430..449
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(623..655)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 gggatcttca taagagaaga gggacagcta tgactgggag tagtcaggag aggaggaaaa
       61 atctggctag taaaacatgt aaggaaaatt ttagggatgt taaagaaaaa aataacacaa
      121 aacaaaatat aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga
      181 cagcaggggg ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag
      241 ccagcatatg ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat
      301 gatttgccct cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg
      361 caatgaaaat aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt
      421 ccccataatt tttggcagag ggaaaaagat ctgctagcta tagttctaga ggtaccggtt
      481 gttgggggct gcaggaattc cgtttttttt tttttttttc ataaaaatta aaaactcaaa
      541 tataattgag gcctctttga gcatggtatc acaagttgat ttggtccaaa catgaagaat
      601 ctgttgtgca ggatttgagt tactttccaa gtcggttcat ctctatgtct gtataaatct
      661 gtcttttctt ggtgtgcttt aatttaatgc aaagatggat accaactctc c
//
