LOCUS       sequence-118306-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for 
            Sf9 expression with a SNAPf tag for fluorescent labeling and a 
            TEV-cleavable His-ZZ tag for purification 
            (His-ZZ-TCS-SNAPf-DYNC1H1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Schlager MA, Hoang HT, Urnavicius L, Bullock SL, Carter AP
  TITLE     In vitro reconstitution of a highly processive recombinant human 
            dynein complex.
  JOURNAL   EMBO J. 2014 Sep 1;33(17):1855-68. doi: 10.15252/embj.201488792. 
            Epub 2014 Jul 1.
  PUBMED    24986880
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201488792"; journalName: "EMBO J"; date: "2014-09-1- 
            1"; volume: "33"; issue: "17"; pages: "1855-68"
COMMENT     Sequence Label: pDyn1
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  195..360
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    481..615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(505..524)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     559..578
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
       61 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttgac
      181 ccaagtcaac cggttgtggg cggacaaaat agttgggaac tgggaggggt ggaaatggag
      241 tttttaagga ttatttaggg aagagtgaca aaatagatgg gaactgggtg tagcgtcgta
      301 agctaatacg aaaattaaaa atgacaaaat agtttggaac tagatttcac ttatctggtt
      361 ccaattagat gggtataccc taggggttat gatagttatt gctcagcggt ggcagcagcc
      421 aactcagctt cctttcgggc tttgttagca gccggatctt ctaggctcaa gcagtgatca
      481 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      541 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      601 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
      661 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      721 cctctagtac ttctcgacaa gcttgtcgag actgcaggct ctagattact cggtgcacag
      781 cacagccaca cctctctcgt agaaagacct gggatcttct ttagtggcga tctcgaagtc
      841 cacggtgaag atcagatcgg ctctggtgaa gttgaggtac acgggcaggg tgaccacgga
      901 ggctttcttc tcagtgtttg tctgcttcac ccatctcagt tgtgtcagtg gcagggcgg
//
