LOCUS       sequence-118339-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GPR153-Tango
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             879..890
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc tcggatccac
      121 tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgcg ccaccatgaa
      181 gacgatcatc gccctgagct acatcttctg cctggtattc gccgactaca aggacgatga
      241 tgacgccagc atcgatatga gcgacgagag gagactgcct ggctctgcgg tggggtggct
      301 ggtatgcggg gggctgtcac tccttgctaa cgcctggggg atcctcagcg tcggagcgaa
      361 acagaagaag tggaagcctc ttgaattcct tctgtgtact cttgcagcaa cccacatgct
      421 gaatgtagca gttcctattg ccacttattc cgtggttcag ttgagaaggc aaaggcccga
      481 ctttgagtgg aatgagggct tgtgtaaggt ttttgtctcc acgttttaca cacttacact
      541 ggccacctgt ttttcagtaa ctagcctgtc ataccaccgg atgtggatgg tatgctggcc
      601 agtcaactat aggcttagta atgctaagaa acaggccgta cacactgtga tgggcatttg
      661 gatggtaagt ttcatacttt ccgctttgcc agccgtgggc tggcatgata ccagtgaacg
      721 gttctataca catgggtgtc ggttcattgt ggccgaaatt ggccttggat tcggcgtgtg
      781 ctttttgctc ttggtcggcg gtagtgtggc catgggagtc atatgtaccg ctatcgccct
      841 gttccagaca ctggccgtgc aggtggggag gcaggccgat cgaagggcgt ttactgtgcc
      901 cactatagta gtggaagacg cacaggggaa aagaa
//
