LOCUS       sequence-11835-e        1428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Morel C, Carlson SM, White FM, Davis RJ
  TITLE     Mcl-1 integrates the opposing actions of signaling pathways that 
            mediate survival and apoptosis.
  JOURNAL   Mol Cell Biol. 2009 Jul . 29(14):3845-52.
  PUBMED    19433446
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2009 Jul . 29(14):3845-52."
COMMENT     Sequence Label: pBabe-Flag hMcl-1
FEATURES             Location/Qualifiers
     source          1..1428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..147
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     163..179
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        251..269
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      304..313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             313..336
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc
       61 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct
      121 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact
      181 ccttctctag gcgccggccg gatcccagtg tggtggtacg taggaattct agcgattacg
      241 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
      301 gccgccacca tggattacaa ggatgacgac gataaggccc gggcggatcc actagtccag
      361 tgtggtggaa ttgcccttat gtttggcctc aaaagaaacg cggtaatcgg actcaacctc
      421 tactgtgggg gggccggctt gggggccggc agcggcggcg ccacccgccc gggagggcga
      481 cttttggcta cggagaagga ggcctcggcc cggcgagaga tagggggagg ggaggccggc
      541 gcggtgattg gcggaagcgc cggcgcaagc cccccgtcca ccctcacgcc agactcccgg
      601 agggtcgcgc ggccgccgcc cattggcgcc gaggtccccg acgtcaccgc gacccccgcg
      661 aggctgcttt tcttcgcgcc cacccgccgc gcggcgccgc ttgaggagat ggaagccccg
      721 gccgctgacg ccatcatgtc gcccgaagag gagctggacg ggtacgagcc ggagcctctc
      781 gggaagcggc cggctgtcct gccgctgctg gagttggtcg gggaatctgg taataacacc
      841 agtacggacg ggtcactacc ctcgacgccg ccgccagcag aggaggagga cgagttgtac
      901 cggcagtcgc tggagattat ctctcggtac cttcgggagc aggccaccgg cgccaaggac
      961 acaaagccaa tgggcaggtc tggggccacc agcaggaagg cgctggagac cttacgacgg
     1021 gttggggatg gcgtgcagcg caaccacgag acggccttcc aaggcatgct tcggaaactg
     1081 gacatcaaaa acgaagacga tgtgaaatcg ttgtctcgag tgatgatcca tgttttcagc
     1141 gacggcgtaa caaactgggg caggattgtg actctcattt cttttggtgc ctttgtggct
     1201 aaacacttga agaccataaa ccaagaaagc tgcatcgaac cattagcaga aagtatcaca
     1261 gacgttctcg taaggacaaa acgggactgg ctagttaaac aaagaggctg ggatgggttt
     1321 gtggagttct tccatgtaga ggacctagaa ggtggcatca ggaatgtgct gctggctttt
     1381 gcaggtgttg ctggagtagg agctggtttg gcatatctaa taagattg
//
