LOCUS       sequence-118350-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, for construction of B-galactosidase protein 
            fusions, frame 3. See Depositor Comments below for information 
            regarding the lacZ sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18-mini-Tn7T-Gm-LacZ30
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(188..235)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(281..367)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(470..564)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(592..608)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(593..609)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg ccacctaaca
       61 attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat tgtcacaacg
      121 ccgcggccaa ttcgatctag aattccattg agtaagtttt taagcacatc agcttcaaaa
      181 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaataggt acttcaagat
      241 ccccaattcg atcgtccggg ccgcaagctc ctagcggcgg atttgtccta ctcaggagag
      301 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      361 ttatttgatg cctcaagcta gagagtcatt accccaggcg tttaagggca ccaataactg
      421 ccttaaaaaa attacgcccc gccctgccac tcatcgcagt ctagcttgga ttctcaccaa
      481 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      541 ttactggatc tatcaacagg agtccaagct cagctaatta agctagctta tcgataccgt
      601 cgacctcgaa ccccacgccc ctctttaata cgacgggcaa tttgcacttc agaaaatgaa
      661 gagtttgctt tagccataac aaaagtccag tatgcttttt cacagcataa ctggactgat
      721 ttcagtttac aactattctg tctagtttaa gactttattg tcatagttta gatctatttt
      781 gttcag
//
