LOCUS       sequence-11836-e        1418 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Morel C, Carlson SM, White FM, Davis RJ
  TITLE     Mcl-1 integrates the opposing actions of signaling pathways that 
            mediate survival and apoptosis.
  JOURNAL   Mol Cell Biol. 2009 Jul . 29(14):3845-52.
  PUBMED    19433446
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2009 Jul . 29(14):3845-52."
COMMENT     Sequence Label: pBabe-Flag hMcl-1(S159A)
FEATURES             Location/Qualifiers
     source          1..1418
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     114..136
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     152..168
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        240..258
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      293..302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             302..325
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc
       61 ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga
      121 acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg
      181 cgccggccgg atcccagtgt ggtggtacgt aggaattcta gcgattacgc caagctcgaa
      241 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgccaccat
      301 ggattacaag gatgacgacg ataagggccc gggcggatcc actagtccag tgtggtggaa
      361 ttgcccttat gtttggcctc aaaagaaacg cggtaatcgg actcaacctc tactgtgggg
      421 gggccggctt gggggccggc agcggcggcg ccacccgccc gggagggcga cttttggcta
      481 cggagaagga ggcctcggcc cggcgagaga tagggggagg ggaggccggc gcggtgattg
      541 gcggaagcgc cggcgcaagc cccccgtcca ccctcacgcc agactcccgg agggtcgcgc
      601 ggccgccgcc cattggcgcc gaggtccccg acgtcaccgc gacccccgcg aggctgcttt
      661 tcttcgcgcc cacccgccgc gcggcgccgc ttgaggagat ggaagccccg gccgctgacg
      721 ccatcatgtc gcccgaagag gagctggacg ggtacgagcc ggagcctctc gggaagcggc
      781 cggctgtcct gccgctgctg gagttggtcg gggaatctgg taataacacc agtacggacg
      841 gggcactacc ctcgacgccg ccgccagcag aggaggagga cgagttgtac cggcagtcgc
      901 tggagattat ctctcggtac cttcgggagc aggccaccgg cgccaaggac acaaagccaa
      961 tgggcaggtc tggggccacc agcaggaagg cgctggagac cttacgacgg gttggggatg
     1021 gcgtgcagcg caaccacgag acggccttcc aaggcatgct tcggaaactg gacatcaaaa
     1081 acgaagacga tgtgaaatcg ttgtctcgag tgatgatcca tgttttcagc gacggcgtaa
     1141 caaactgggg caggattgtg actctcattt cttttggtgc ctttgtggct aaacacttga
     1201 agaccataaa ccaagaaagc tgcatcgaac cattagcaga aagtatcaca gacgttctcg
     1261 taaggacaaa acgggactgg ctagttaaac aaagaggctg ggatgggttt gtggagttct
     1321 tccatgtaga ggacctagaa ggtggcatca ggaatgtgct gctggctttt gcaggtgttg
     1381 ctggagtagg agctggtttg gcatatctaa taagattg
//
