LOCUS       sequence-118362-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 
            / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: PA-mCherry-miniSOG-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             255..575
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     polyA_signal    697..818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(734..753)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     788..807
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cggatgtacc ccgaggaggc gccctgaagg gcgaggtcaa gcccagagtg aagctgaagg
       61 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
      121 tgcccggcgc ctacaacgtc aaccgcaagc tggacatcac cagccacaac gaggactaca
      181 ccatcgtgga gcagtacgag agagccgagg gccgccactc caccggcggc atggacgagc
      241 tgtacaaggg cggcatggag aagagcttcg tgatcaccga cccccgcctg cccgacaacc
      301 ccatcatctt cgccagcgac ggcttcctgg agctgaccga gtacagcaga gaggagatcc
      361 tgggccgcaa cggccgcttc ctgcagggcc ccgagaccga ccaggccacc gtgcagaaga
      421 tccgcgacgc catccgcgac cagcgcgaga tcaccgtgca gctgatcaac tacaccaaga
      481 gcggcaagaa gttctggaac ctgctgcacc tgcagcccat gcgcgaccag aagggcgagc
      541 tgcagtactt catcggcgtg cagctggacg gctaagcggc cgcgactcta gatcataatc
      601 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
      661 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
      721 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
      781 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
      841 tattttgtta aaattcgcgt taaatttttg tttaatcagc tcattttt
//
