LOCUS       sequence-118363-         478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-p300 Core
FEATURES             Location/Qualifiers
     source          1..478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgttcagctt tcgttaaatt atcaaactta cgttgagtga ttaacttggc gtttagaagt
       61 tgtctccaat agtttttcat ctttttgact acttcttcac ttggaacgtt atccgattta
      121 ccacgatttt tatcagaacg cgttaagacc ttattgtcta ttgaatcgtc tttaaggaaa
      181 ctttgtggaa caatggcatc gacatcataa tcacttaaac gattaatatc taattcttgg
      241 tccacataca tgtctcttcc attttggaga taatagagat agagcttttc attttgcaat
      301 tgagtatttt caacaggatg ctctttaaga atctgacttc ctaattcttt gataccttct
      361 tcgattcgtt tcatacgctc tcgcgaattt ttctggccct tttgagttgt ctgattttca
      421 cgtgccattt caataacgat attttctggc ttatgccgcc ccattacttt gaccaatt
//
