LOCUS       sequence-11837-e        1422 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Morel C, Carlson SM, White FM, Davis RJ
  TITLE     Mcl-1 integrates the opposing actions of signaling pathways that 
            mediate survival and apoptosis.
  JOURNAL   Mol Cell Biol. 2009 Jul . 29(14):3845-52.
  PUBMED    19433446
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2009 Jul . 29(14):3845-52."
COMMENT     Sequence Label: pBabe-Flag hMcl-1(T163A)
FEATURES             Location/Qualifiers
     source          1..1422
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     119..141
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     157..173
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        245..263
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      298..307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             307..330
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc
       61 aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc
      121 cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct
      181 ctaggcgccg gccggatccc agtgtggtgg tacgtaggaa ttctagcgat tacgccaagc
      241 tcgaaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgcc
      301 accatggatt acaaggatga cgacgataag gcccgggcgg atccactagt ccagtgtggt
      361 ggaattgccc ttatgtttgg cctcaaaaga aacgcggtaa tcggactcaa cctctactgt
      421 gggggggccg gcttgggggc cggcagcggc ggcgccaccc gcccgggagg gcgacttttg
      481 gctacggaga aggaggcctc ggcccggcga gagatagggg gaggggaggc cggcgcggtg
      541 attggcggaa gcgccggcgc aagccccccg tccaccctca cgccagactc ccggagggtc
      601 gcgcggccgc cgcccattgg cgccgaggtc cccgacgtca ccgcgacccc cgcgaggctg
      661 cttttcttcg cgcccacccg ccgcgcggcg ccgcttgagg agatggaagc cccggccgct
      721 gacgccatca tgtcgcccga agaggagctg gacgggtacg agccggagcc tctcgggaag
      781 cggccggctg tcctgccgct gctggagttg gtcggggaat ctggtaataa caccagtacg
      841 gacgggtcac taccctcggc gccgccgcca gcagaggagg aggacgagtt gtaccggcag
      901 tcgctggaga ttatctctcg gtaccttcgg gagcaggcca ccggcgccaa ggacacaaag
      961 ccaatgggca ggtctggggc caccagcagg aaggcgctgg agaccttacg acgggttggg
     1021 gatggcgtgc agcgcaacca cgagacggcc ttccaaggca tgcttcggaa actggacatc
     1081 aaaaacgaag acgatgtgaa atcgttgtct cgagtgatga tccatgtttt cagcgacggc
     1141 gtaacaaact ggggcaggat tgtgactctc atttcttttg gtgcctttgt ggctaaacac
     1201 ttgaagacca taaaccaaga aagctgcatc gaaccattag cagaaagtat cacagacgtt
     1261 ctcgtaagga caaaacggga ctggctagtt aaacaaagag gctgggatgg gtttgtggag
     1321 ttcttccatg tagaggacct agaaggtggc atcaggaatg tgctgctggc ttttgcaggt
     1381 gttgctggag taggagctgg tttggcatat ctaataagat tg
//
