LOCUS       sequence-118371-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA in Leishmania.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Zhang WW, Matlashewski G
  TITLE     CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e00861-15. doi: 10.1128/mBio.00861-15.
  PUBMED    26199327
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e00861-15. doi"; pages: " 
            10.1128/mBio.00861-15"
COMMENT     Sequence Label: pSPneoHHgRNAH
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           193..229
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        254..329
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           336..403
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(579..598)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gaggtctgcg attgacgtag gagttgcaag ggggaggggg tatgaacggg gtgggtagag
       61 cttttttagg tggaagtagt gagagggtgg gcttgtgaga atttgaggtg tgttcgtgat
      121 gtgtggatct tatcgggggc tcggttgagt ttttggtttg gtgattttgt aggaagctta
      181 agatctcacc atctgatgag tccgtgagga cgaaacgagt aagctcgtca tggtggggtc
      241 ttcgagaaga cctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      301 aacttgaaaa agtggcaccg agtcggtgct tttttggccg gcatggtccc agcctcctcg
      361 ctggcgccgg ctgggcaaca tgcttcggca tggcgaatgg gacggatccc cgggtaccga
      421 gctctctctt ctccttcccc tctctctcct ctctcctctc tccagagctc tctcttctcc
      481 ttcccctctc tctcctctct cctctctcca gagctcgaat tcgcatgatt gaacaagatg
      541 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
      601 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
      661 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
      721 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
      781 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
      841 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
      901 ttgatccggc tacctgcc
//
