LOCUS       sequence-118374-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bleomycin drug selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Zhang WW, Matlashewski G
  TITLE     CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e00861-15. doi: 10.1128/mBio.00861-15.
  PUBMED    26199327
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e00861-15. doi"; pages: " 
            10.1128/mBio.00861-15"
COMMENT     Sequence Label: pSPble
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             267..641
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(711..730)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(712..730)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(817..834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcgactctag aggatccccg gccgccttgt gcggctcagc ggaggcgctg agtgcgagca
       61 ccgccgcgac gcacaccagc aacaccacaa gcggacgcac gctgcggatc ttcatcggat
      121 cgggtaccga gctctctctt ctccttcccc tctctctcct ctctcctctc tccagagctc
      181 tctcttctcc ttcccctctc tctcctctct cctctctcca gagctcgaat tcatcgatga
      241 tatcagatcc ccaactagat atcaccatgg ccaagttgac cagtgccgtt ccggtgctca
      301 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
      361 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
      421 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
      481 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
      541 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
      601 ccggcaactg cgtgcacttc gtggccgagg agcaggactg accgacgccg accaacaccg
      661 ccggtccgac gcggcccgac gggtccgagg cctcggagat ctgccggtct ccctatagtg
      721 agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg
      781 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      901 agaatcaggg
//
