LOCUS       sequence-118384-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation:448 / 473, Emission:500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: miniSOG-Membrane-7
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      142..151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             148..468
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     polyA_signal    590..711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(627..646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     681..700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
       61 gccaccatgc tgtgctgtat gagaagaacc aaacaggttg aaaagaatga tgaggaccaa
      121 aagatcgcgg atccaccggt cgccaccatg gagaaaagtt tcgtgataac tgatccacgg
      181 ctgccagaca atcccatcat cttcgcatcc gatggcttcc tggagctgac cgagtattcc
      241 agagaggaga tcctgggccg caatggccgc tttctgcagg gaccagagac agaccaggcc
      301 acagtgcaga agattcgcga tgccattaga gatcagcgcg agattaccgt gcagctgata
      361 aactacacaa aaagcgggaa gaaattctgg aacctcctgc acctccagcc catgagggac
      421 cagaagggtg agctccagta tttcatcgga gtgcagctgg atggataagc ggccgcgact
      481 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
      541 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
      601 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      661 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
      721 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
      781 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag acc
//
