LOCUS       sequence-11840-e        1451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Morel C, Carlson SM, White FM, Davis RJ
  TITLE     Mcl-1 integrates the opposing actions of signaling pathways that 
            mediate survival and apoptosis.
  JOURNAL   Mol Cell Biol. 2009 Jul . 29(14):3845-52.
  PUBMED    19433446
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2009 Jul . 29(14):3845-52."
COMMENT     Sequence Label: pCDNA3.1-hMcl-1(T163A)
FEATURES             Location/Qualifiers
     source          1..1451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     71..90
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        71..89
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1275..1316
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1326..1343
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1366..1383)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
       61 ttatcgaaat taatacgact cactataggg agacccaagc tggctagtta agcttggtac
      121 cgagctcgga tccactagtc cagtgtggtg gaattgccct tatgtttggc ctcaaaagaa
      181 acgcggtaat cggactcaac ctctactgtg ggggggccgg cttgggggcc ggcagcggcg
      241 gcgccacccg cccgggaggg cgacttttgg ctacggagaa ggaggcctcg gcccggcgag
      301 agataggggg aggggaggcc ggcgcggtga ttggcggaag cgccggcgca agccccccgt
      361 ccaccctcac gccagactcc cggagggtcg cgcggccgcc gcccattggc gccgaggtcc
      421 ccgacgtcac cgcgaccccc gcgaggctgc ttttcttcgc gcccacccgc cgcgcggcgc
      481 cgcttgagga gatggaagcc ccggccgctg acgccatcat gtcgcccgaa gaggagctgg
      541 acgggtacga gccggagcct ctcgggaagc ggccggctgt cctgccgctg ctggagttgg
      601 tcggggaatc tggtaataac accagtacgg acgggtcact accctcggcg ccgccgccag
      661 cagaggagga ggacgagttg taccggcagt cgctggagat tatctctcgg taccttcggg
      721 agcaggccac cggcgccaag gacacaaagc caatgggcag gtctggggcc accagcagga
      781 aggcgctgga gaccttacga cgggttgggg atggcgtgca gcgcaaccac gagacggcct
      841 tccaaggcat gcttcggaaa ctggacatca aaaacgaaga cgatgtgaaa tcgttgtctc
      901 gagtgatgat ccatgttttc agcgacggcg taacaaactg gggcaggatt gtgactctca
      961 tttcttttgg tgcctttgtg gctaaacact tgaagaccat aaaccaagaa agctgcatcg
     1021 aaccattagc agaaagtatc acagacgttc tcgtaaggac aaaacgggac tggctagtta
     1081 aacaaagagg ctgggatggg tttgtggagt tcttccatgt agaggaccta gaaggtggca
     1141 tcaggaatgt gctgctggct tttgcaggtg ttgctggagt aggagctggt ttggcatatc
     1201 taataagatt gggcaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
     1261 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     1321 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     1381 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     1441 ccactcccac t
//
